Figure 6.

(A) Dendrogram depicting phylogenetic relationships among 14 different chalcone synthase isoforms. The numbers above the branches indicate the bootstrap confidence value. The scale bar shows the number of substitutions per amino acid. Bootstrap analysis of 500 replicates was performed. (B) Alignment of partial sequences of the deduced amino acid sequences of chalcone synthase genes (CHS1, CHS2, and CHS3) for milk thistle available in the GenBank databases based on the amino sequences. The red box indicates cysteine conservation. In the lowest line ‘*’ indicates full conservation among all sequences, ‘:’ shows highly conservative exchanges and ‘.’ indicates conservative exchanges of amino acids.