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Determination of hamamelitannin in whISOBAX (WH) by reverse phase HPLC analysis. WH (StaphOff Biotech Inc) or HAMA (Sigma-Aldrich) were applied to Durashell reverse phase C18 column in water containing 0.1% trifluoroacetic acid (TFA). Bound material was eluted with an acetonitrile gradient. The amount of HAMA in WH was determined by comparing the retention time and absorbance spectrum with the HAMA standard.
