Figure 5.

The effect of GT and WH on pre-formed S. epidermidis biofilm; Cells were grown in microtiter plates with slight shaking for 4 hrs. Unbound cells were removed and bound cells (biofilm cells) were further incubated overnight with increasing concentrations of GT, WH, or a combination of the two extracts. Unbound cells were removed. Remaining attached (biofilm) bacteria were washed and stained with crystal violet, and their OD determined. As a control for single extract treatment of GT or WH, cells were grown in TSB alone. As a control for combination treatments of GT+WH, cells were grown with no GT but with WH at 1:26 or 1:40 dilutions.