| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| FITC anti-mouse KRLG1 | TONBO biosciences | Cat# 35-5893; RRID:AB_2621718 |
| FITC anti-mouse IFN gamma | TONBO Biosciences | Cat# 35-7311-U100; RRID:AB_2621724 |
| FITC anti-mouse CD4 | TONBO Biosciences | Cat# 35-0031-U100; RRID:AB_2621665 |
| FITC anti-mouse CD8a | BioLegend | Cat# 100706; RRID:AB_312745 |
| FITC anti-mouse FOXP3 | eBioscience | Cat# 11-5773-82; RRID:AB_465243 |
| PE anti-mouse CTLA-4 | eBioscience | Cat# 12-1522-82; RRID:AB_465243 |
| PE anti-mouse F4/80 | eBioscience | Cat# 12-4801-82; RRID:AB_465923 |
| PE anti-mouse CD4 | BioLegend | Cat# 100407; RRID:AB_312692 |
| PE anti-mouse CD8a | eBioscience | Cat# 12-0081-81; RRID:AB_465529 |
| PE anti-mouse FOXP3 | TONBO bioscience | Cat# 50-5773-U100; RRID:AB_2621797 |
| PE anti-mouse CD274 | eBioscience | Ca# 12-5982-81; RRID:AB_466088 |
| PE anti-human/mouse CD44 | eBioscience | Cat# 12-0441-81; RRID:AB_465663 |
| PerCP-Cy5.5 anti-mouse Ki67 | BioLegend | Cat# 652424; RRID:AB_2629531 |
| PerCP-Cy5.5 anti-mouse Ly6G | BioLegend | Cat# 127616; RRID:AB_1727563 |
| PeCy7 anti-mouse Granzyme B | eBioscience | Cat# 25-8898-80; RRID:AB_10853338 |
| PeCy7 anti-mouse GITR | BD Pharmigen | Cat# 558140; RRID:AB_647252 |
| PeCy7 anti-mouse CD11b | BD Pharmigen | Cat# 552850; RRID:AB_394491 |
| PeCy7 anti-mouse NKp46 | eBioscience | Cat# 25-3351-82; RRID:AB_394491 |
| PeCy7 anti-mouse CD8a | eBioscience | Cat# 25-0081-81; RRID:AB_469583 |
| PeCy7 anti-mouse CD4 | TONBO bioscience | Cat# 60-0041-U100; RRID:AB_469583 |
| PeCy7 anti-mouse CD80 | eBioscience | Cat# 25-0801-82; RRID:AB_2573370 |
| eFluor450 anti-mouse IL-2 | eBioscience | Cat# 48-7021-82; RRID:AB_1944462 |
| vFluor450 anti-mouse CD4 | TONBO bioscience | Cat# 75-0042-U100; RRID:AB_2621928 |
| eFluor450 anti-mouse CD8a | eBioscience | Cat# 48-0081-82; RRID:AB_1272198 |
| BV605 anti-mouse Ly6C | BioLegend | Cat# 128036; RRID:AB_2562353 |
| BV605 anti-mouse CXCR3 | BioLegend | Cat# 126523; RRID:AB_2561353 |
| BV650 anti-mouse CD8a | eBioscience | Cat# 100555; RRID:AB_2561353 |
| BV650 anti-mouse CD4 | BioLegend | Cat# 100555; RRID:AB_2562529 |
| BV650 anti-mouse CD11b | BioLegend | Cat# 101259; RRID:AB_2566568 |
| BV711 anti-mouse CD11b | BioLegend | Cat# 101242; RRID:AB_2563310 |
| BV711 anti-mouse CD4 | BioLegend | Cat# 100557; RRID:AB_2562607 |
| BV711 anti-mouse CD8a | BioLegend | Cat# 100759; RRID:AB_2563510 |
| BV785 anti-mouse MHCII I-A/I-E | BioLegend | Cat# 107646; RRID:AB_313317 |
| BV785 anti-mouse B220 | BioLegend | Cat# 103246; RRID:AB_2563256 |
| APC anti-mouse CD4 | eBioscience | Cat# 17-0042-82; RRID:AB_469323 |
| APC anti-mouse FOXP3 | eBioscience | Cat# 20-0191-U100; RRID:AB_2621561 |
| APC anti-mouse MHCI H-2Kd,H-2Dd | eBioscience | Cat# 17-5998-82; RRID:AB_2573250 |
| APC anti-mouse TNF alpha | eBioscience | Cat# 506308; RRID:AB_315429 |
| APC anti-mouse CD25 | eBioscience | Cat# 20-0251-U100; RRID:AB_2621567 |
| APC anti-mouse CD86 | eBioscience | Cat# 17-0862-81; RRID:AB_469418 |
| AlexaFluor647 anti-mouse CCR2 (CD192) | BioLegend | Cat# 150604; RRID:AB_2566140 |
| Vinculin mouse monoclonal | Santa Cruz Biotechnology | Cat# SC-73614; RRID:AB_1131294 |
| p53 mouse monoclonal antibody | Cell Signaling Technology | Cat# 2524; RRID:AB_331743 |
| InVivoPlus mouse IgG2a isotype control | BioXCell | Cat# BP0085; RRID:AB_1107771 |
| InVivo monoclonal anti mouse CSF1R | BioXCell | Cat#BE0213; RRID:AB_466565 |
| InVivo monoclonal anti-CD25 | BioXCell | Cat# BE0012; RRID:AB_1107619 |
| Anti-mouse CD28 Functional grade purified | eBioscience | Cat# 16-0281-86; RRID:AB_468923 |
| Anti-mouse CD3e Functional grade purified | eBioscience | Cat# 16-0031-86; RRID:AB_468849 |
| IRDye® 800CW Donkey anti-mouse | Li-Cor | Cat# 926-32212; RRID:AB_621847 |
| IRDye® 680LT Donkey anti-Rabbit | Li-Cor | Cat# 926-68023; RRID:AB_10706167 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Murine Il-2 | Peprotech | Cat# 212-12 |
| Ovalbumin peptide 257-364 | Francis Crick Institute | N/A |
| Ovalbumin peptide 323-339 | Francis Crick Institute | N/A |
| Recombinant Murine M-CSF | Peprotech | Cat# 315-02 |
| Critical Commercial Assays | ||
| Mouse M-CSF ELISA Kit | RayBio ® | Cat# ELM-MCSF |
| Mouse G-CSF ELISA Kit | RayBio ® | Cat# ELM-GCSF |
| Mouse CXCL1/KC ELISA Kit | OriGene Techologies, Inc | Cat# EA100460 |
| Mouse Cytokine Array Panel A - Proteome Profiler | R&D systems ® | Cat# ARY006 |
| Mouse CCL2 Uncoated ELISA Kit | Invitrogen | Cat# 99-7391-22 |
| Mouse MIP1 alpha (CCL3) Uncoated ELISA Kit | Theromo Fisher Scientific | Cat# 88-56013-22 |
| Mouse LIX ELISA Kit | RayBio ® | Cat# ELM-LIX |
| ProcartaPlex Mouse Cytokine & Chemokine Panel 1 (26 plex) | Invitrogen eBioscience | EPX260-26088-901 |
| LEGEND MAX Mouse CCL11 ELISA Kit | BioLegend | Cat# 4438907 |
| High capacity cDNA reversts transcription kit | AppliedBiosystems | Cat# 4368814 |
| RNeasy ® Mini Kit | QIAGEN | Cat# 157029548 |
| RNase Free and DNase set | QIAGEN | Cat# 79254 |
| Deposited Data | ||
| PAAD - TCGA | TCGA | https://gdac.broadinstitute.org/; RRID:SCR_003193 |
| Experimental Models: Cell Lines | ||
| Phoenix-ECO | ATCC® | Cat# CRL-3214; RRID:CVCL_H717 |
| KC1 Pdx1-cre; LSL-KRasG12D | Beatson Institute for Cancer Research | N/A |
| KC3 Pdx1-cre; LSL-KRasG12D | Beatson Institute for Cancer Research | N/A |
| KC3 Pdx1-cre; LSL-KRasG12D | Beatson Institute for Cancer Research | N/A |
| KFC1 Pdx1-cre; LSL-KrasG12D; Trp53fl/+ | Beatson Institute for Cancer Research | N/A |
| KFC2 Pdx1-cre; LSL-KrasG12D; Trp53fl/+ | Beatson Institute for Cancer Research | N/A |
| KFC3 Pdx1-cre; LSL-KrasG12D; Trp53fl/+ | Beatson Institute for Cancer Research | N/A |
| ID8 | Walton et al., 2016 | N/A |
| ID8-p53KO | Walton et al., 2016 | N/A |
| iKRAS;p53f/+ | Ying et al., 2012 | N/A |
| Experimental Models: Organisms/Strains | ||
| C57Bl6/J mice females (6-10weeks) | Charles River Laboratories | CR: 632 |
| CD1 nude- Crl:CD1-Foxn1nu./nu. females (6-10weeks) | Charles River Laboratories | CR: 087 |
| FVB/NCrl females (6-10weeks) | Charles River Laboratories | CR: 207 |
| Oligonucleotides | ||
| Primers for qPCR T cell cytokines See Table 1 | This paper | N/A |
| Trp53 gRNA/Cas9 | Walton et al., 2016 | N/A |
| Recombinant DNA | ||
| pBABE-iRFP plasmid | Hock et al., 2014 | N/A |
| pCl-neo-mOVA | Yang et al., 2010 | Addgene Cat# 25099; RRID:Addgene_25099 |
| MIGR1 | Pear et al., 1998 | Addgene Cat# 27490; RRID:Addgene_27490 |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/; RRID:SCR_001935 |
| Image Studio v5 | Li-Cor | N/A |
| FlowJo version 9.0 | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads; RRID:SCR_008520 |
| survival package version 2.41-3 | https://cran.r-project.org/web/packages/surviva/index.html | |
| R version 3.4.3 | RStudio | http://www.R-project.org; RRID:SCR_000432 |
| Prism Version 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
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