Antibodies |
|
FITC anti-mouse KRLG1 |
TONBO biosciences |
Cat# 35-5893; RRID:AB_2621718
|
FITC anti-mouse IFN gamma |
TONBO Biosciences |
Cat# 35-7311-U100; RRID:AB_2621724
|
FITC anti-mouse CD4 |
TONBO Biosciences |
Cat# 35-0031-U100; RRID:AB_2621665
|
FITC anti-mouse CD8a |
BioLegend |
Cat# 100706; RRID:AB_312745
|
FITC anti-mouse FOXP3 |
eBioscience |
Cat# 11-5773-82; RRID:AB_465243
|
PE anti-mouse CTLA-4 |
eBioscience |
Cat# 12-1522-82; RRID:AB_465243
|
PE anti-mouse F4/80 |
eBioscience |
Cat# 12-4801-82; RRID:AB_465923
|
PE anti-mouse CD4 |
BioLegend |
Cat# 100407; RRID:AB_312692
|
PE anti-mouse CD8a |
eBioscience |
Cat# 12-0081-81; RRID:AB_465529
|
PE anti-mouse FOXP3 |
TONBO bioscience |
Cat# 50-5773-U100; RRID:AB_2621797
|
PE anti-mouse CD274 |
eBioscience |
Ca# 12-5982-81; RRID:AB_466088
|
PE anti-human/mouse CD44 |
eBioscience |
Cat# 12-0441-81; RRID:AB_465663
|
PerCP-Cy5.5 anti-mouse Ki67 |
BioLegend |
Cat# 652424; RRID:AB_2629531
|
PerCP-Cy5.5 anti-mouse Ly6G |
BioLegend |
Cat# 127616; RRID:AB_1727563
|
PeCy7 anti-mouse Granzyme B |
eBioscience |
Cat# 25-8898-80; RRID:AB_10853338
|
PeCy7 anti-mouse GITR |
BD Pharmigen |
Cat# 558140; RRID:AB_647252
|
PeCy7 anti-mouse CD11b |
BD Pharmigen |
Cat# 552850; RRID:AB_394491
|
PeCy7 anti-mouse NKp46 |
eBioscience |
Cat# 25-3351-82; RRID:AB_394491
|
PeCy7 anti-mouse CD8a |
eBioscience |
Cat# 25-0081-81; RRID:AB_469583
|
PeCy7 anti-mouse CD4 |
TONBO bioscience |
Cat# 60-0041-U100; RRID:AB_469583
|
PeCy7 anti-mouse CD80 |
eBioscience |
Cat# 25-0801-82; RRID:AB_2573370
|
eFluor450 anti-mouse IL-2 |
eBioscience |
Cat# 48-7021-82; RRID:AB_1944462
|
vFluor450 anti-mouse CD4 |
TONBO bioscience |
Cat# 75-0042-U100; RRID:AB_2621928
|
eFluor450 anti-mouse CD8a |
eBioscience |
Cat# 48-0081-82; RRID:AB_1272198
|
BV605 anti-mouse Ly6C |
BioLegend |
Cat# 128036; RRID:AB_2562353
|
BV605 anti-mouse CXCR3 |
BioLegend |
Cat# 126523; RRID:AB_2561353
|
BV650 anti-mouse CD8a |
eBioscience |
Cat# 100555; RRID:AB_2561353
|
BV650 anti-mouse CD4 |
BioLegend |
Cat# 100555; RRID:AB_2562529
|
BV650 anti-mouse CD11b |
BioLegend |
Cat# 101259; RRID:AB_2566568
|
BV711 anti-mouse CD11b |
BioLegend |
Cat# 101242; RRID:AB_2563310
|
BV711 anti-mouse CD4 |
BioLegend |
Cat# 100557; RRID:AB_2562607
|
BV711 anti-mouse CD8a |
BioLegend |
Cat# 100759; RRID:AB_2563510
|
BV785 anti-mouse MHCII I-A/I-E |
BioLegend |
Cat# 107646; RRID:AB_313317
|
BV785 anti-mouse B220 |
BioLegend |
Cat# 103246; RRID:AB_2563256
|
APC anti-mouse CD4 |
eBioscience |
Cat# 17-0042-82; RRID:AB_469323
|
APC anti-mouse FOXP3 |
eBioscience |
Cat# 20-0191-U100; RRID:AB_2621561
|
APC anti-mouse MHCI H-2Kd,H-2Dd |
eBioscience |
Cat# 17-5998-82; RRID:AB_2573250
|
APC anti-mouse TNF alpha |
eBioscience |
Cat# 506308; RRID:AB_315429
|
APC anti-mouse CD25 |
eBioscience |
Cat# 20-0251-U100; RRID:AB_2621567
|
APC anti-mouse CD86 |
eBioscience |
Cat# 17-0862-81; RRID:AB_469418
|
AlexaFluor647 anti-mouse CCR2 (CD192) |
BioLegend |
Cat# 150604; RRID:AB_2566140
|
Vinculin mouse monoclonal |
Santa Cruz Biotechnology |
Cat# SC-73614; RRID:AB_1131294
|
p53 mouse monoclonal antibody |
Cell Signaling Technology |
Cat# 2524; RRID:AB_331743
|
InVivoPlus mouse IgG2a isotype control |
BioXCell |
Cat# BP0085; RRID:AB_1107771
|
InVivo monoclonal anti mouse CSF1R |
BioXCell |
Cat#BE0213; RRID:AB_466565
|
InVivo monoclonal anti-CD25 |
BioXCell |
Cat# BE0012; RRID:AB_1107619
|
Anti-mouse CD28 Functional grade purified |
eBioscience |
Cat# 16-0281-86; RRID:AB_468923
|
Anti-mouse CD3e Functional grade purified |
eBioscience |
Cat# 16-0031-86; RRID:AB_468849
|
IRDye® 800CW Donkey anti-mouse |
Li-Cor |
Cat# 926-32212; RRID:AB_621847
|
IRDye® 680LT Donkey anti-Rabbit |
Li-Cor |
Cat# 926-68023; RRID:AB_10706167
|
|
Chemicals, Peptides, and Recombinant Proteins |
|
Recombinant Murine Il-2 |
Peprotech |
Cat# 212-12 |
Ovalbumin peptide 257-364 |
Francis Crick Institute |
N/A |
Ovalbumin peptide 323-339 |
Francis Crick Institute |
N/A |
Recombinant Murine M-CSF |
Peprotech |
Cat# 315-02 |
|
Critical Commercial Assays |
|
Mouse M-CSF ELISA Kit |
RayBio ® |
Cat# ELM-MCSF |
Mouse G-CSF ELISA Kit |
RayBio ® |
Cat# ELM-GCSF |
Mouse CXCL1/KC ELISA Kit |
OriGene Techologies, Inc |
Cat# EA100460
|
Mouse Cytokine Array Panel A - Proteome Profiler |
R&D systems ® |
Cat# ARY006 |
Mouse CCL2 Uncoated ELISA Kit |
Invitrogen |
Cat# 99-7391-22 |
Mouse MIP1 alpha (CCL3) Uncoated ELISA Kit |
Theromo Fisher Scientific |
Cat# 88-56013-22 |
Mouse LIX ELISA Kit |
RayBio ® |
Cat# ELM-LIX |
ProcartaPlex Mouse Cytokine & Chemokine Panel 1 (26 plex) |
Invitrogen eBioscience |
EPX260-26088-901 |
LEGEND MAX Mouse CCL11 ELISA Kit |
BioLegend |
Cat# 4438907 |
High capacity cDNA reversts transcription kit |
AppliedBiosystems |
Cat# 4368814 |
RNeasy ® Mini Kit |
QIAGEN |
Cat# 157029548 |
RNase Free and DNase set |
QIAGEN |
Cat# 79254 |
|
Deposited Data |
|
PAAD - TCGA |
TCGA |
https://gdac.broadinstitute.org/; RRID:SCR_003193
|
|
Experimental Models: Cell Lines |
|
Phoenix-ECO |
ATCC® |
Cat# CRL-3214; RRID:CVCL_H717 |
KC1 Pdx1-cre; LSL-KRasG12D
|
Beatson Institute for Cancer Research |
N/A |
KC3 Pdx1-cre; LSL-KRasG12D
|
Beatson Institute for Cancer Research |
N/A |
KC3 Pdx1-cre; LSL-KRasG12D
|
Beatson Institute for Cancer Research |
N/A |
KFC1 Pdx1-cre; LSL-KrasG12D; Trp53fl/+
|
Beatson Institute for Cancer Research |
N/A |
KFC2 Pdx1-cre; LSL-KrasG12D; Trp53fl/+
|
Beatson Institute for Cancer Research |
N/A |
KFC3 Pdx1-cre; LSL-KrasG12D; Trp53fl/+
|
Beatson Institute for Cancer Research |
N/A |
ID8 |
Walton et al., 2016 |
N/A |
ID8-p53KO
|
Walton et al., 2016 |
N/A |
iKRAS;p53f/+
|
Ying et al., 2012 |
N/A |
|
Experimental Models: Organisms/Strains |
|
C57Bl6/J mice females (6-10weeks) |
Charles River Laboratories |
CR: 632 |
CD1 nude- Crl:CD1-Foxn1nu./nu. females (6-10weeks) |
Charles River Laboratories |
CR: 087 |
FVB/NCrl females (6-10weeks) |
Charles River Laboratories |
CR: 207 |
|
Oligonucleotides |
|
Primers for qPCR T cell cytokines See Table 1 |
This paper |
N/A |
Trp53 gRNA/Cas9 |
Walton et al., 2016 |
N/A |
|
Recombinant DNA |
|
pBABE-iRFP plasmid |
Hock et al., 2014 |
N/A |
pCl-neo-mOVA |
Yang et al., 2010 |
Addgene Cat# 25099; RRID:Addgene_25099 |
MIGR1 |
Pear et al., 1998 |
Addgene Cat# 27490; RRID:Addgene_27490 |
|
Software and Algorithms |
|
ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/; RRID:SCR_001935
|
Image Studio v5 |
Li-Cor |
N/A |
FlowJo version 9.0 |
FlowJo |
https://www.flowjo.com/solutions/flowjo/downloads; RRID:SCR_008520
|
survival package version 2.41-3 |
|
https://cran.r-project.org/web/packages/surviva/index.html |
R version 3.4.3 |
RStudio |
http://www.R-project.org; RRID:SCR_000432
|
Prism Version 7 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798
|