Figure 2.

Non-specific Loss of Predator Aversion in T. gondii-Infected Mice

(A) Schematic of bobcat aversion test with example traces from a mouse infected with T. gondii.

(B) Quantification of behaviors during bobcat aversion test. Bobcat aversion index is the (time spent in the open compartment with bobcat odor)/(time spent in open compartment without bobcat odor). Positive values signify increased presence in open compartment when the odor is present.

(C) Schematic of predator avoidance test in a 4-chamber arena containing two predator odors (fox and bobcat), a non-predator odor (guinea pig), and the odor of the mouse (home). Representative trace from a T. gondii ME49-infected mouse.

(D and E) Quantification of total investigation times (D) and investigation times of each odor source (E).

(F) Schematic of live predator avoidance test in a two-chamber arena with a hideaway and an exposed compartment containing an anesthetized rat. Representative traces from a T. gondii ME49-infected mouse.

(G) Quantification of percentage of time spent in the exposed compartment before and after introduction of the rat.

Bars indicate mean ± SEM, and each dot represents an individual. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. For details of the statistical analyses, see Table S1.