Figure 4.

Immune Cells and Vasculature of the Murine and Human HGSOC

(A) Proportions of immune cell populations estimated from murine tumors and human omental metastases using CIBERSORT. 30200, HGS2, and HGS3, n = 4; 60577 and HGS4, n = 5; HGS1, n = 3; human omental tumors, n = 9. Median values are depicted.

(B) Flow cytometric analysis of the immune infiltrate of peritoneal tumors, close to endpoint, from mice injected with 60577, 30200, or HGS cell lines. B cells: CD45⁺ CD19⁺, CD4 cells: CD45⁺ CD3⁺ CD4⁺, CD8 cells: CD45⁺ CD3⁺ CD8⁺, monocytes: CD45⁺ CD11b⁺ Ly6C⁺, macrophages: CD45⁺, CD11b⁺ F4/80⁺ (Ly6C/G⁻), granulocytes: CD45⁺ CD11b⁺ Ly6G⁺, CD11c⁺ cells: CD45⁺ CD11b⁺ CD11c⁺ (F4/80⁻ Ly6C/G⁻). A similar analysis of the immune infiltrate in the diseased omentum from patients who underwent upfront surgery is shown for comparison (HuHGS).

(C) Quantification of the number of CD3⁺ cells/mm² by IHC on peritoneal tumors from mice injected with 60577, 30200, or HGS cell lines. Quantification of CD3⁺ cells in biopsies from patients (HuHGS) is shown for comparison. Representative images are shown; scale bars set to 100 μm.

(D) Quantification of the percentage of an area positive for F4/80 by IHC on peritoneal tumors from mice injected with 60577, 30200, or HGS cell lines. Quantification of CD68⁺ area in patient biopsies is included for comparison. Representative images are shown; scale bars set to 100 μm.

(E) IHC for endomucin (mouse) and CD31 (human) staining, quantified using the Definiens Tissue Studio platform with the blood vessel detection feature. Each dot represents a tumor from an individual mouse or human. Representative images for HGS2 and HuHGS are depicted at left. Scale bars, 100 μm.

(F) Heatmap illustrating Gene Ontology biological process angiogenesis gene expression across mouse models and human omental tumors.