REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-mouse CD45-BV785 (Clone 30-F11)	Biolegend	Cat# 103149; RRID:AB_2564590
Armenian hamster anti-mouse CD3-PE-Cy7 (Clone 145-2C11)	Biolegend	Cat# 100320; RRID:AB_312685
Rat anti mouse CD4-BV605	Biolegend	Cat#100548; RRID:AB_2563054
Rat anti-mouse CD8a-APC (Clone 53-6.7)	eBioscience	Cat# 17-0081-83; RRID:AB_469336)
Rat anti-mouse/human CD11b-BV650 (Clone M1/70)	Biolegend	Cat# 101239; RRID:AB_11125575)
Armenian hamster anti-mouse CD11c-FITC, (Clone N418)	eBioscience	Cat#11-0114-82; RRID:AB_464940
Rat anti-mouse F4/80-PE (Clone BM8)	Biolegend	Cat#123110; RRID:AB_893486
Rat anti-mouse Ly6C -eFluor450 (Clone HK1.4)	eBioscience	Cat#48-5932-82; RRID:AB_10805519
Rat anti-mouse Ly6G(Gr1)-AF700 (Clone RB6-BC5)	eBioscience	Cat#56-5931-82; RRID:AB_10805519
Rat anti-mouse CD19 PerCP-Cy5.5 (Clone 6D5)	eBioscience	Cat#115534; RRID:AB_2072925
Rat anti-mouse I-A/I-E- APC-Cy7 (Clone M5/114)	Biolegend	Cat#107628; RRID:AB_2069377
InVivoMAb anti-mouse IL-6	BioXCell	Cat#BE0046; RRID:AB_1107709
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase	BioXCell	Cat# BE0088; RRID:AB_1107775
F(ab’)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary HRP	Thermo Fisher	Cat# A24555; RRID:AB_2536023
Goat anti-rabbit biotinylated IgG	Vector Laboratories	Cat# BA-1000; RRID:AB_2313606
Anti-rat IgG	Vector Laboratories	Cat# BA-4001; RRID:AB_10015300
Anti-mouse IgG	Vector Laboratories	Cat# BA-2000; RRID:AB_2313581
Rat monoclonal anti-CD3	Abcam	Cat# ab11089; RRID: AB_369097
Rabbit polyclonal anti-human CD3	Agilent	Cat# A0452; RRID: AB_2335677
Rat monoclonal anti-F4/80	Serotec/BioRad	Cat# MCA497; RRID:AB_2098196
Rat monoclonal anti-endomucin (V.7C7)	Santa Cruz Biotechnology	Cat# sc-65495; RRID:AB_2100037
Mouse monoclonal anti-PECAM-1 (0.N.100)	Santa Cruz Biotechnology	Cat# sc-71872; RRID:AB_1125653)
Rabbit polyclonal anti-actin, smooth muscle	Abcam	Cat# ab5694; RRID:AB_2223021
Mouse monoclonal anti-actin, smooth muscle Clone 1A4	Sigma-Aldrich	Cat# A2547; RRID:AB_476701
Rabbit monoclonal anti-Ki67 [SP6]	Abcam	Cat#ab16667; RRID:AB_302459)
Rabbit polyclonal anti-VCAN	Sigma-Aldrich	Cat# HPA004726; RRID:AB_1080561)
Rabbit polyclonal anti-COL11A1	Sigma-Aldrich	Cat#HPA052246; RRID:N/A
Rabbit polyclonal anti-COL11A1	Novus	Cat# NBP2-58159; RRID:N/A
Rabbit polyclonal anti-COL1A1	Abcam	Cat# ab21286; RRID:AB_446161)
Rabbit polyclonal anti-FN1	Sigma-Aldrich	Cat# F3648; RRID:AB_476976
Rabbit polyclonal anti-CTSB	Novus	Cat# NBP1-19797; RRID:AB_2086951
Rabbit polyclonal anti-COMP	GeneTex	Cat# GTX14515; RRID:AB_845475)
Rabbit monoclonal anti-Runx2 [EPR14334]	Abcam	Cat# ab192256; RRID:AB_2713945
Rabbit monoclonal anti-Vitamin D3Receptor (D2K6W)	Cell Signaling Technology	Cat#12550; RRID:AB_2637002
Rabbit monoclonal anti-Folate Binding Protein EPR20277	Abcam	Cat# ab221543; RRID: N/A
TruStain fcX (anti-mouse CD16/32) Antibody	Biolegend	Cat#101320; RRID:AB_1574975
Biological Samples
Human HGSOC omental metastasis samples	Barts Health NHS Trust, St. George’s University Hospitals NHS Foundation Trust and Barts Gynae Tissue Bank	(https://directory.biobankinguk.org/Profile/Biobank/GBR-1-128) HTA license number 12199 (REC no: 10/H0304/14 and 15/EE/0151)
Chemicals, Peptides, and Recombinant Proteins
collagenase from Clostridium histolyticum	Sigma-Aldrich	Cat# C9263
DNase I from bovine pancreas	Sigma-Aldrich	Cat# D4513
HBSS (10X), no calcium, no magnesium, no phenol red	GIBCO	Cat# 14185-045
Fixable Viability Dye eFluor® 506	eBioscience	Cat# v65-0866-18
Formalin solution neutral buffered 10%	Sigma Aldrich	Cat# HT501128
Xylene, Technical, Fisher Chemical	Fisher Scientific	Cat# X/0100/17
Ethanol, 99.8%, as ethanol,anhydrous,(denat. with 2% IPA + 2% MEK)	Fisher Scientific	Cat#12367103
Hydrogen Peroxide 30-32% (w/w) (100 Volumes), Certified AR for Analysis	Fisher Scientific	Cat# H/1800/15
Methanol 99.9% (GLC) 0.7915 g/mL for analysis CertiFied AR	Fisher Scientific	Cat# M/4000/PB17
Antibody diluent	Zytomed Systems	Cat# ZUC025-100
Diaminobenzidine substrate-chromogen (Dako Liquid DAB+ Substrate Chromogen System)	Dako	Cat# K3468
Gill’s hematoxylin I	Sigma-Aldrich	Cat# GHS1128
DPX Mountant for histology	Sigma-Aldrich	Cat# 06522
Bouin’s Solution	Sigma Aldrich	Cat# HT10132
Weigert’s Iron Hematoxylin Solution	Sigma Aldrich	Cat# HT1079
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)	Abcam	Cat# ab93684
Novocastra protein block	Leica Biosystem	Cat# RE7102-CE
Bovine Serum Albumin	Sigma Aldrich	Cat# A4503
Goat serum	Sigma Aldrich	Cat# G9023
Phosphate-buffered saline (PBS) (1x)	GIBCO	Cat# 141-90-094
Proteinase K in TE-CaCl2 buffer pH8	Invitrogen	Cat# 25530015
Tween 20	Sigma-Aldrich	Cat# P7949-500ml
Antigen Unmasking Solution, Citric Acid Based	Vector Laboratories	Cat# H3300
Triton X-100	Sigma-Aldrich	Cat#T8787
Protein Block, Serum-Free	DAKO Agilent	Cat# X0909
EDTA 0.5M	Thermo fisher	Cat# AM9262
Doxycycline hyclate	Sigma-Aldrich	Cat# D9891
DMEM:F12 1:1 with GlutaMax	GIBCO	Cat# 31331-028
Fetal Bovine Serum	Hyclone	Cat# 11521831
Penicillin-Streptomycin (10,000 U/mL)	Invitrogen	Cat# 15140-122
Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS-A)	Invitrogen	Cat# 51300
Hydrocortisone, γ-irradiated, powder	Sigma	Cat# H0135
Murine Epidermal Growth Factor (EGF)	Sigma	Cat# E4127
Antibiotic-Antimycotic (100X)	GIBCO	Cat# 5240-062
0.5% Trypsin-EDTA (10X)	GIBCO	Cat#15400-054
Carboplatin	Hospira	Cas#41575-94-4
Fixable Viability Dye eFluor506	eBioscience	Cat# 65-0866-14
Collagenase type I	Fisher Scientific	Cat# 17018-029
Glacial acetic acid	Fisher Scientific	Cat# 10021123
Critical Commercial Assays
Trichrome Stain (Masson) Kit	Sigma-Aldrich	Cat# HT15-1KT
anti-rat ImmPRESS-HRP reagent (MP7444, Vector	Vector Laboratories	Cat# MP7444
ImmPress HRP anti-rabbit (MP7451, Vector)	Vector Laboratories	Cat# MP7451
Super Sensitive Polymer HRP IHC	BioGenex	Cat# QD430-XAKE
RNAlater-ice	Thermo Fisher Scientific	Cat# 4427575
RNeasy Mini kit	Quiagen	Cat# 74104
RNA NanoChips	Agilent	Cat# 5067-1511
QiaShredders	Quiagen	Cat# 79654
RNeasy Plus Mini kit	Quiagen	Cat# 74134
DNeasy Blood and Tissue kit	Quiagen	Cat# 69504
Deposited Data
Murine and human omental metastasis data	GEO	GSE132289
Experimental Models: Cell Lines
60577	Laboratory of Difilippantonio S.	Szabova et al., 2014
30200	Laboratory of Difilippantonio S.	Szabova et al., 2014
HGS1	This paper	N/A
HGS2	This paper	N/A
HGS3	This paper	N/A
HGS4	This paper	N/A
Experimental Models: Organisms/Strains
PAX8-rtTA, TetO-Cre mice	Laboratory of Ronny Drapkin	Perets et al., 2013
TP53fl/fl [B6.129P2-Trp53tm1Brn/J] mice	Jackson Laboratory	N/A
PTENfl/fl mice	Laboratory of Bart Vanhaesebroeck	Groszer et al., 2001
BrCa2fl/fl mice	Laboratory of Ashok Venkitaraman	Jonkers et al., 2001
FVB mice	Charles River	N/A
C57/Bl6J mice	Charles River	N/A
Software and Algorithms
FlowJo software, Tree Star, Inc.	FlowJo Software for Mac Version 10.Ashland	https://www.flowjo.com/solutions/flowjo
NPD.view 2.7.25 software		https://www.hamamatsu.com/eu/en/product/type/U12388-01/index.html
ImageJ 1.48v image processing and analysis program (NIH, Bethesda, MD) with color threshold and color deconvolution plug-ins	Schneider et al., 2012, Ruifrok and Johnston, 2001	https://imagej.nih.gov/ij/ and https://imagej.net/Colour_Deconvolution
Definiens® software	(Definiens AG, Germany)	http://www.astrazeneca.com
Graphpad Prism, San Diego, CA	NA	https://www.graphpad.com
R v3.5.1	NA	http://www.R-project.org
KCsmart	Bioconductor	de Ronde et al., 2010
DNACopy	Bioconductor	Seshan and Olshen, 2019
HTSeq	https://htseq.readthedocs.io/en/release_0.11.1/	Anders et al., 2015
EdgeR	Bioconductor	Robinson et al., 2010
limma	Bioconductor	Ritchie et al., 2015
DAVID v6.8	https://david.ncifcrf.gov	Huang et al., 2009
Clipper	Bioconductor	Martini et al., 2013
GSVA	Bioconductor	Hänzelmann et al., 2013
siggenes	Bioconductor	Schwender, 2019
biomaRt	Bioconductor	Durinck et al., 2009
factoextra	CRAN	https://cran.r-project.org/web/packages/factoextra/index.html
dnet	CRAN	https://cran.r-project.org/web/packages/dnet/index.html
ggplot2	CRAN	https://cran.r-project.org/web/packages/ggplot2/index.html
WGCNA	CRAN	Langfelder and Horvath, 2008
classyfire	CRAN	Chatzimichali and Bessant, 2016
Other
BD LSR Fortessa cytometer	BD	N/A
Hamamatsu NanoZoomer S210 Slide Scanner	N/A	N/A
3DHISTECH Panoramic 250 digital slide scanner	3DHISTECH, Hungary	N/A
70 μm strainers	Fisher Scientific	N/A
GentleMACS M Tubes	Miltenyi	Cat# 130-093-236
Primaria flasks	Corning	Cat# 353808
Instron ElectroPuls E1000	Instron, UK	N/A