Figure 2.

Classical swine fever virus (CSFV) genome detection by quantitative real-time RT-PCR (qRT-PCR) after sample treatment for inactivation of CSFV. Four infectious cell culture supernatants and four infectious serum samples were 1:2 diluted in phosphate buffered saline (PBS)-Tween₂₀ (PBS 0.05% Tween₂₀ for cell culture supernatant and PBS 0.3% Tween₂₀ for serum) and incubated at 56 °C for 30 min (treated). As a control for viral genome load, samples were 1:2 diluted in PBS and not incubated at 56 °C for 30 min (PBS control). All samples were analyzed in duplicates in a CSFV-specific qRT-PCR and the mean values are shown.