Table 4.
Effect of Tre on O2•− generation rate, H2O2, MDA, and LOX activity in leaves of maize seedlings under saline and low P stress. Each value of data represents the mean of three independent experiments, each replicated three times. Values within a column with different letters are significant at p ≤ 0.05 applying the LSD test.
| Source of Variation | O2•− (nmol g−1 FW min−1) |
H2O2 (µmol g−1 FW) |
MDA (nmol g−1 FW)) |
LOX (µmol min−1 mg−1 Protein) |
|---|---|---|---|---|
| Treatment | ||||
| Control (C) | 2.93 d | 5.91 d | 24.1 f | 13.3 e |
| Saline (S) | 6.77 a | 11.8 b | 44.0 cd | 31.0 bc |
| S + Tre | 3.77 c | 8.63 c | 34.0 e | 22.6 d |
| Low P (LP) | 5.00 b | 11.8 b | 51.1 b | 34.2 b |
| LP + Tre | 3.74 c | 7.05 d | 42.3 d | 26.8 cd |
| S + LP | 7.28 a | 15.4 a | 64.7 a | 47.1 a |
| S + LP + Tre | 5.27 b | 10.9 b | 48.4 bc | 31.7 b |
| SE | 0.35 | 0.58 | 3.03 | 2.19 |
| F ratio (df = 6, 112) | 43.5 | 62.3 | 36.2 | 45.2 |
| Variety | ||||
| BHM-7 | 4.73 b | 9.78 b | 52.9 a | 31.5 a |
| BHM-9 | 5.19 a | 10.64 a | 35.3 b | 27.5 b |
| SE | 1.90 | 0.31 | 1.62 | 1.18 |
| F ratio (df = 1, 112) | 6.17 | 7.73 | 117.7 | |
| Interaction | ||||
| C × BHM-7 | 2.84 | 6.2 | 25.5 g | 14.9 |
| C × BHM-9 | 3.02 | 5.7 | 22.8 g | 11.8 |
| S × BHM-7 | 7.16 | 11.5 | 53.4 c | 33.9 |
| S × BHM-9 | 6.37 | 12.1 | 34.6 d-f | 28 |
| S + Tre × BHM-7 | 3.37 | 8.2 | 40.9 d | 22.6 |
| S + Tre × BHM-9 | 4.16 | 9 | 27.2 fg | 22.6 |
| LP × BHM-7 | 4.86 | 10.7 | 66.1 ab | 36.2 |
| LP × BHM-9 | 5.13 | 12.9 | 36.1 de | 32.1 |
| LP + Tre × BHM-7 | 3.48 | 7.1 | 54.6 c | 30.1 |
| LP + Tre × BHM-9 | 3.99 | 7 | 29.9 eg | 23.5 |
| S + LP × BHM-7 | 6.52 | 14.6 | 71.7 a | 52 |
| S + LP × BHM-9 | 8.03 | 16.2 | 57.7 bc | 42.2 |
| S + LP + Tre × BHM-7 | 4.89 | 10.2 | 57.9 bc | 30.7 |
| S + LP + Tre × BHM-9 | 5.65 | 11.6 | 38.9 d | 32.6 |
| SE | 0.98 | 0.83 | 4.28 | 3.11 |
| F ratio (df= 6, 112) | 2.05 | 1.39 | 4.14 | 1.65 |