Figure 1.

Fluorescent microscopy of phage binding to SKOV 3 cells. (a) Cells were incubated with 10¹¹ V/mL pJ18, pJ24, wild type (WT), or dimethyl sulfoxide (DMSO) (vehicle) and probed by a biotinylated anti-Fd filamentous phage antibody and streptavidin-fluorescein isothiocyanate (FITC). Cells were further stained with ProLong™ Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) and detected using a Keyence BZ X800 fluorescent microscope (Keyence, Itasca, IL, USA). (b) The corrected total cell fluorescence was determined using ImageJ software, and showed that pJ18 and pJ24 exhibited higher fluorescence compared to WT and DMSO. Phage pJ18 and pJ24 also showed significantly higher fluorescence compared to DMSO (not shown). A p-value of <0.05 was considered significant. *** p < 0.001. Green (FITC-phage); blue (DAPI).