Figure 3.

Binding of phage to ovarian carcinoma (SKOV-3) cells. Cells were grown to 80% confluency on 96-well plates, fixed using 0.2% Triton X-100 and 10% formalin, and then incubated with 10⁹ or 10¹⁰ V/mL pJ18, pJ24, or WT phage. Phage binding was probed by biotinylated anti-Fd filamentous phage antibody and streptavidin-HRP, and detected spectrophotometrically at 405 nM after addition of 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). Binding of phage pJ18 and pJ24 to SKOV-3 cells was significantly higher at 10¹⁰ V/mL compared to WT. Measurements were performed on a SpectraMax 250 microplate reader (Molecular Devices, San Jose, CA, USA). A p-value of <0.05 was considered significant. * p < 0.05.