Figure 5.

The effect of the three treatment conditions: NaOCl, ethylenediaminetetraacetic acid (EDTA) and NaOCl + EDTA on pre-formed 24 h biofilms viability in relation to untreated biofilms. Biofilms were treated for 5 min and reincubated with fresh RPMI. XTT readings were taken at 0, 24, 48 72 h. Values were plotted as log₁₀ on the Y axis. (a) absorbance values for LBF (b) absorbance values for HBF. Statistical significance between the three treatment conditions at 48, and 72 h was presented as * p < 0.05, ** p < 0.01 and **** p < 0.0001. Whereas the significant difference in relation to untreated biofilms of the same treatment condition over time was presented as ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 and ^#### p < 0.0001.