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. 2019 Nov 20;8(12):600. doi: 10.3390/foods8120600

Table 2.

Milk clotting indexes (MCIs) obtained for C. cardunculus exctract (Cc), C. papaya (Cp), Calf rennet (Cr) and F. carica (Fc) latexes, in bovine, buffalo, goat and sheep milks, at different incubation temperatures (25, 37, 50, 60 and 70 °C).

Coagulants Temperature (°C) Milk Samples
Bovine Buffalo Goat Sheep
Cc 25 3.47 dC (±0.25) 16.10 bB (±2.01) 12.49 cB (±2.50) 35.04 bA (±2.00)
37 5.46 γB (±0.34) 10.61 βB (±1.51) 8.33 χB (±2.08) 25.24 bA (±3.03)
50 17.51 hD (±0.27) 24.11 hB (±2.01) 18.41 hB (±3.08) 41.40 hA (±3.50)
60 21.17 ηD (±0.14) 73.28 ηC (±3.04) 110.33 ηA (±10.02) 87.72 ηB (±3.04)
70 11.21 xC (±0.252) 36.21 xB (±2.03) 37.39 xB (±5.05) 82.31 xA (±3.05)
Cp 25 23.38 cB (±0.12) N/A 49.32 bA (±4.04) N/A
37 39.83 βB (±0.24) N/A 88.96 βA (±4.57) N/A
50 3.65 jB (±0.15) N/A 6.40 hA (±0.26) 3.24 kB(±0.31)
60 10.10 φB (±0.10) N/A 10.12 φA (±3.01) 4.71 κB (±0.30)
70 3.52 zB (±0.23) N/A 6.53 xA (±2.50) 2.23 yB (±0.30)
Cr 25 314.17 aD (±1.04) 862.92 aA (±20.63) 371.92 aC (±10.54) 716.74 aB (±30.53)
37 327.62 αC (±2.51) 596.61 αAB (±25.15) 551.67 αB (±25.17) 625.09 αA (±25.00)
50 447.75 gB (±2.54) 515.43 gA (±15.02) 244.86 gC (±15.00) 540.93 gA (±40.03)
60 2311.78 γA (±7.60) 878.43 γD (±25.70) 1428.47 γC (±42.40) 1839.26 γB (±40.02)
70 2532.74 wA (±16.06) 200.00 wC (±25.17) 1564.46 wA (±41.08) 1528.77 wA (±30.08)
Fc 25 56.42 bA (±2.13) N/A N/A 19.82 cB (±3.02)
37 24.26 χA (±2.05) N/A N/A 23.05 βA (±3.00)
50 19.90 hB (±1.65) 28.68 hA (±3.51) 1.14 jC (±0.21) 21.18 jB (±2.02)
60 13.23 ηφB (±2.04) 27.32 φA (±3.05) 2.90 φC (±0.40) 16.46 φB (±2.50)
70 18.08 xB (±2.00) 35.51 xA (±3.12) 5.63 yB (±0.35) 14.96 yB (±3.00)

Results are mean values of triplicate experiments with standard deviations. Values with different lowercase letters differ significantly (Tukey’s test, p < 0.05) considering the coagulant factor for the single milk animal source incubated at each temperature (25 °C (a, b, c, d), 37 °C (α, β, γ, δ), 50 °C (g, h, j, k), 60 °C (γ, η, φ, κ), 70 °C (w, x, y, z)). Values with different capital letters (A, B, C, D) differ significantly (Tukey’s test, p < 0.05) according to milk factor at each coagulant. N/A: not available (coagulation has not been revealed).