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. 2019 Nov 27;8(4):268. doi: 10.3390/pathogens8040268

Figure 5.

Figure 5

miR-19b-3p mimic pretreatment contributes to neuroinflammatory responses in meningitic E. coli-challenged mice. (A) qPCR analysis of miR-19b-3p in brain lysates from control mice, PCN033-challenged mice with or without miR-19b-3p mimic pretreatment. U6 was used as the internal reference. Data are presented as mean ± SD from five individual mice. (B) The mRNA level of TNFAIP3 in mouse brains from control mice, PCN033-challenged mice with or without miR-19b-3p mimic pretreatment was analyzed by qPCR. GAPDH was used as the internal reference. Data were presented as mean ± SD from five individual mice. The protein level of TNFAIP3 in mouse brains from control mice, PCN033-challenged mice with or without miR-19b-3p mimic pretreatment was additionally analyzed by Western blot. β-actin was used as the loading control. (C) qPCR analysis of TNF-α, IL-1β, IL-6, and CCL2 transcription from control mice, PCN033-challenged mice with or without miR-19b-3p mimic pretreatment. GAPDH was used as the internal reference. Data are presented as mean ± SD from five individual mice. (D) ECL analysis of TNF-α, IL-1β, IL-6, and CCL2 levels in brain lysates from control mice, PCN033-challenged mice with or without miR-19b-3p mimic pretreatment. Data are expressed as mean ± SD (n = 5). * p < 0.05; ** p < 0.01.