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. 2019 Dec 10;7(4):212. doi: 10.3390/vaccines7040212

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative two-dimensional electrophoresis (2DE) proteome and immunome of the secretome (A–D) and total extract (E–H) from L. prolificans. Secreted proteins resolved by 2DE and Coomassie Brilliant Blue (CBB) stained (A,C) or electrotransferred to PVDF membranes to detect immunoreactive proteins recognized by mice inoculated with the contact (B) and infective dose (D). Total extract proteins resolved by 2DE and CBB stained (E,G) or electrotransferred to PVDF membranes to detect immunoreactive proteins recognized by mice inoculated with the contact (F) and infective dose (H).