Table 1.
Identification by LC-MS/MS of immunodominant antigens from Lomentospora prolificans secretome reacting with serum IgG of mice inoculated with the contact and infective dose of this fungus.
| Spot No. | NCBI No. | Protein Name | Microorganism | Matching Peptides | Sequence Coverage (%) | MASCOT Score | Theor. pI/Mr (kDa) | Exper. pI/Mr (kDa) |
|---|---|---|---|---|---|---|---|---|
| Secreted antigens of L. prolificans reacting with serum of mice inoculated with the contact dose (Figure 4A) | ||||||||
| 1 | - | 5.62/46.98 | ||||||
| 2 | PKS06692.1 | Adenosylhomocysteinase (hp jhhlp_006766) | L. prolificans | 1 | 5 | 156 | 5.77/49.09 | 5.79/46.61 |
| 3 | - | 5.90/46.24 | ||||||
| Secreted antigens of L. prolificans reacting with serum of mice inoculated with the infective dose (Figure 4C) | ||||||||
| 4 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 27 | 51 | 1758 | 5.11/71.50 | 5.16/76.79 |
| 5 | PKS07974.1 | Haloacid dehalogenase-like Hydrolase (hp jhhlp_006586) | L. prolificans | 4 | 35 | 179 | 4.93/26.36 | 4.57/30.28 |
| 6 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 5 | 7 | 170 | 5.11/71.50 | 4.25/18.45 |
| 7 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 5 | 8 | 160 | 5.11/71.50 | 4.29/18.45 |
| 8 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 5 | 8 | 172 | 5.11/71.50 | 4.36/18.41 |
| 9 | PKS07141.1 | Glycosyl hydrolase family 16 protein (Hp jhhlp_005741) | L. prolificans | 2 | 7 | 90 | 4.44/41.13 | 4.43/18.43 |
| AAC49800.1 | Glyceraldehyde-3-phosphate dehydrogenase | C. albicans | 1 | 8 | 59 | 6.33/35.51 | ||
| 10 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 1 | 1 | 54 | 5.11/71.50 | 4.35/17.95 |
| 11 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 3 | 5 | 131 | 5.11/71.50 | 4.50/16.83 |
| PKS12714.1 | Nuclear movement protein nudC (hp jhhlp_000922) | L. prolificans | 1 | 5 | 81 | 5.30/21.12 | ||
| 12 | PKS12714.1 | Nuclear movement protein nudC (hp jhhlp_000922) | L. prolificans | 2 | 6 | 108 | 5.30/21.12 | 4.82/16.18 |
| 13 | PKS06713.1 | Hp jhhlp_006787 | L. prolificans | 1 | 7 | 107 | 4.82/22.77 | 4.42/14.90 |
| 14 | PKS07725.1 | Cerato-platanin (Hp jhhlp_006333) | L. prolificans | 2 | 23 | 256 | 5.37/14.03 | 4.79/13.45 |