Table 2.
Identification by LC-MS/MS of immunodominant antigens from Lomentospora prolificans total extract reacting with serum IgG of mice inoculated with the contact and infective dose of this fungus.
| Spot No. | NCBI No. | Protein Name | Microorganism | Matching Peptides | Sequence Coverage (%) | MASCOT Score | Theor. pI/Mr (kDa) | Exper. pI/Mr (kDa) |
|---|---|---|---|---|---|---|---|---|
| Antigens of L. prolificans total extract reacting with serum of mice inoculated with the contact dose (Figure 4E) | ||||||||
| 1 | PKS10679.1 | Proteasome subunit alpha (hp jhhlp_002435) | L. prolificans | 5 | 22 | 304 | 5.28/28.04 | 4.73/28.35 |
| PKS08308.1 | NADP oxidoreductase coenzyme F420-dependent (hp jhhlp_005252) | L. prolificans | 5 | 27 | 288 | 5.66/32.60 | ||
| 2 | PKS10679.1 | Proteasome subunit alpha (hp jhhlp_002435) | L. prolificans | 10 | 41 | 474 | 5.28/28.04 | 4.91/28.32 |
| Antigens of L. prolificans total extract reacting with serum of mice inoculated with the infective dose (Figure 4G) | ||||||||
| 3 | PKS06544.1 | Dipeptidyl-peptidase (hp jhhlp_007292) | L. prolificans | 13 | 20 | 602 | 5.19/79.99 | 5.26/81.64 |
| 4 | PKS06544.1 | Dipeptidyl-peptidase (hp jhhlp_007292) | L. prolificans | 15 | 23 | 797 | 5.19/79.99 | 5.32/81.10 |
| 5 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 22 | 49 | 1658 | 5.11/71.50 | 4.08/83.83 |
| 6 | PKS06780.1 | Heat shock 70 kDa protein (hp jhhlp_006854) | L. prolificans | 23 | 43 | 1159 | 5.11/71.50 | 4.66/77.14 |
| 7 | PKS10466.1 | Proteasomal ubiquitin receptor (hp jhhlp_002217) | L. prolificans | 5 | 25 | 225 | 4.65/40.76 | 4.62/51.74 |
| 8 | PKS12946.1 | Carboxypeptidase (hp jhhlp_000287) | L. prolificans | 2 | 4 | 111 | 4.99/60.56 | 4.66/51.68 |
| 9 | PKS13321.1 | RAN-specific GTPase-activating protein (hp jhhlp_000092) | L. prolificans | 9 | 40 | 607 | 5.11/28.07 | 4.76/35.25 |
| PKS12026.1 | 60S acidic ribosomal protein P0 (hp jhhlp_001322) | L. prolificans | 4 | 20 | 374 | 4.81/33.75 | ||
| 10 | PKS10574.1 | Proliferating cell nuclear antigen (hp jhhlp_002328) | L. prolificans | 9 | 39 | 449 | 4.56/28.92 | 4.36/32.09 |
| 11 | PKS10574.1 | Proliferating cell nuclear antigen (hp jhhlp_002328) | L. prolificans | 3 | 18 | 326 | 4.56/28.92 | 4.41/32.01 |
| 12 | PKS10574.1 | Proliferating cell nuclear antigen (hp jhhlp_002328) | L. prolificans | 2 | 11 | 118 | 4.56/28.92 | 4.45/32.18 |
| XP_016642844.1 | NAC domain-containing protein (hp SAPIO_CDS5122) | S. apiospermum | 2 | 13 | 100 | 4.59/22.31 | ||
| 13 | PKS07974.1 | Haloacid dehalogenase-like hydrolase (hp jhhlp_006586) | L. prolificans | 5 | 31 | 390 | 4.93/26.36 | 4.51/30.62 |
| 14 | PKS07974.1 | Haloacid dehalogenase-like hydrolase (hp jhhlp_006586) | L. prolificans | 7 | 41 | 431 | 4.93/26.36 | 4.41/30.00 |
| 15 | PKS10466.1 | Proteasomal ubiquitin receptor (hp jhhlp_002217) | L. prolificans | 3 | 12 | 174 | 4.65/40.76 | 4.57/30.19 |
| PKS11928.1 | Eukaryotic translation initiation factor 6 (hp jhhlp_001223) | L. prolificans | 4 | 25 | 154 | 4.33/23.13 | ||
| 16 | PKS10466.1 | Proteasomal ubiquitin receptor (hp jhhlp_002217) | L. prolificans | 4 | 12 | 241 | 4.65/40.76 | 4.57/29.58 |
| PKS11928.1 | Eukaryotic translation initiation factor 6 (hp jhhlp_001223) | L. prolificans | 4 | 26 | 214 | 4.33/23.13 | ||
| 17 | PKS12778.1 | Vacuolar protein sorting-associated protein 28 (hp jhhlp_000989) | L. prolificans | 4 | 19 | 157 | 4.68/27.98 | 4.64/29.58 |
| PKS09814.1 | Aminopeptidase (hp jhhlp_004437) | L. prolificans | 3 | 7 | 129 | 5.50/60.03 | ||
| 18 | PKS09755.1 | NFU1 iron-sulfur cluster scaffold-like protein (hp jhhlp_004376) | L. prolificans | 5 | 28 | 299 | 5.78/33.19 | 4.61/24.42 |
| 19 | PKS09755.1 | NFU1 iron-sulfur cluster scaffold-like protein (hp jhhlp_004376) | L. prolificans | 6 | 31 | 343 | 5.78/33.19 | 4.64/28.20 |