Table 2.
Relative quantification of chemical amino acid modifications (LC-MS peptide mapping).
| Chemical Mod. (%) | Trastuzumab 1 | RM | Degly | Man5 | G0F | G2F | G2S2F ST3 | G2S2F ST6 |
|---|---|---|---|---|---|---|---|---|
| LC N30 2 deamidation | 9.6 | 8.7 | 10.9 | 9.1 | 9.1 | 9.0 | 9.6 | 10.0 |
| LC N30 2 succinimide | 0.6 | 0.7 | 0.7 | 0.6 | 0.8 | 0.7 | 1.1 | 1.1 |
| HC N54 2 deamidation | 1.5 | 1.6 | 1.7 | 2.2 | 1.7 | 2.0 | 1.8 | 1.6 |
| HC N54 2 succinimide | 3.9 | 4.0 | 3.9 | 4.1 | 3.9 | 3.9 | 3.7 | 3.8 |
| HC D98 2 isomerization | 7.7 | 7.6 | 8.0 | 10.0 | 7.0 | 7.7 | 7.9 | 8.7 |
| HC D98 2 succinimide | 3.5 | 4.1 | 3.6 | 2.5 | 4.3 | 3.8 | 4.3 | 4.3 |
| HC N389/390 3 deam. | 2.0 | 1.9 | 2.2 | 1.6 | 1.9 | 1.9 | 2.1 | 2.0 |
| HC N389/390 3 succ. | 1.7 | 1.7 | 1.7 | 2.0 | 1.8 | 1.7 | 1.8 | 1.8 |
| HC M252 3 oxidation | 2.3 | 2.6 | 3.4 | 5.2 | 3.0 | 3.8 | 3.8 | 3.8 |