Table 1.
Proteins identified in pellicle, GCF, and saliva samples
| No | Accession number | Score | %Cov | Name | Peptides (95%) |
|---|---|---|---|---|---|
| 1 | P02768 | 132.13 | 85.1 | Serum albumin | 160 |
| 2 | P19013 | 124.83 | 88 | Keratin, type II cytoskeletal 4 | 148 |
| 3 | P13646 | 118.77 | 78.6 | Keratin, type I cytoskeletal 13 | 186 |
| 4 | P02538 | 112.25 | 71.8 | Keratin, type II cytoskeletal 6A | 118 |
| 5 | P02788 | 93.78 | 76.3 | Lactotransferrin | 65 |
| 6 | P01833 | 84.02 | 59.4 | Polymeric immunoglobulin receptor | 90 |
| 7 | P04745 | 79.37 | 81.2 | Alpha-amylase 1 | 129 |
| 8 | P15924 | 77.69 | 33.2 | Desmoplakin | 44 |
| 9 | P02533 | 81.99 | 83.7 | Keratin, type I cytoskeletal 14 | 75 |
| 10 | P02812 | 67.59 | 88.9 | Basic salivary proline-rich protein 2 | |
| 11 | P01876 | 64.9 | 91.2 | Ig alpha-1 chain C region | 101 |
| 12 | P13647 | 99.44 | 70.5 | Keratin, type II cytoskeletal 5 | 88 |
| 13 | P02787 | 56.01 | 58.6 | Serotransferrin | 31 |
| 14 | P04083 | 46.39 | 75.7 | Annexin A1 | 36 |
| 15 | Q9HC84 | 45.57 | 10.1 | Mucin-5B | 23 |
| 16 | O60437 | 45.31 | 28.6 | Periplakin | 25 |
| 17 | P01036 | 41.83 | 72.3 | Cystatin-S | 119 |
| 18 | P08779 | 80.32 | 79.1 | Keratin, type I cytoskeletal 16 | 67 |
| 19 | P63261 | 37.63 | 80.3 | Actin, cytoplasmic 2 | 33 |
| 20 | Q9UBC9 | 37.18 | 93.5 | Small proline-rich protein 3 | 20 |
| 21 | P01871 | 36.8 | 59.5 | Ig mu chain C region | 26 |
| 22 | P25311 | 35.97 | 67.1 | Zinc-alpha-2-glycoprotein | 26 |
| 23 | P04280 | 66.34 | 88.5 | Basic salivary proline-rich protein 1 | 62 |
| 24 | P05164 | 34.63 | 48.5 | Myeloperoxidase | 23 |
| 25 | P02810 | 34.35 | 72.3 | Salivary acidic proline-rich phosphoprotein | 77 |
| 26 | P13645 | 53.41 | 63.2 | Keratin, type I cytoskeletal 10 | 42 |
| 27 | P06733 | 34 | 69.8 | Alpha-enolase | 23 |
| 28 | P14618 | 33.39 | 52 | Pyruvate kinase isozymes M1/M2 | 19 |
| 29 | P31947 | 31.24 | 76.6 | 14-3-3 protein sigma | 19 |
| 30 | P22079 | 30.42 | 43 | Lactoperoxidase | 17 |
| 31 | Q09666 | 29.74 | 24.3 | Neuroblast differentiation-associated protein | 21 |
| 32 | P07355 | 29.14 | 54 | Annexin A2 | 17 |
| 33 | P61626 | 28.07 | 63.5 | Lysozyme C | 47 |
| 34 | Q9UBG3 | 26.93 | 54.1 | Cornulin | 25 |
| 35 | Q9UGM3 | 26.74 | 37.9 | Deleted in malignant brain tumors 1 protein | 16 |
| 36 | P01857 | 26.64 | 79.1 | Ig gamma-1 chain C region | 29 |
| 37 | P05109 | 26 | 90.3 | Protein S100-A8 | 23 |
| 38 | Q92817 | 25.4 | 21.5 | Envoplakin | 13 |
| 39 | P12273 | 24.91 | 78.1 | Prolactin-inducible protein | 30 |
| 40 | Q01546 | 41.09 | 51.7 | Keratin, type II cytoskeletal 2 | 31 |
| 41 | P01024 | 23.14 | 19.4 | Complement C3 | 18 |
| 42 | A8K2U0 | 22.13 | 22.3 | Alpha-2-macroglobulin-like protein 1 | 14 |
| 43 | Q08188 | 21.57 | 38.1 | Protein-glutamine gamma-glutamyltransferase E | 12 |
| 44 | P04406 | 21.56 | 58.5 | Glyceraldehyde-3-phosphate dehydrogenase | 15 |
| 45 | P06702 | 21.49 | 91.2 | Protein S100-A9 | 38 |
| 46 | P14923 | 19.71 | 34.8 | Junction plakoglobin | 14 |
| 47 | Q8N1N4 | 31.48 | 43.1 | Keratin, type II cytoskeletal 78 | 20 |
| 48 | P08107 | 19.12 | 19.5 | Heat shock 70 kDa protein 1A/1B | 10 |
| 49 | P09228 | 31.05 | 76.6 | Cystatin-SA OS = Homo sapiens | 73 |
| 50 | P07476 | 18.23 | 32.5 | Involucrin OS = Homo sapiens | 12 |
The top 50 proteins identified in the mixed sample of pellicles, GCF, parotid saliva, and mixed saliva are listed. During iTRAQ analysis, a mixture of the four samples was analyzed by LC–MS/MS quantitatively. All of the major proteins in Fig. 3b except for histone H2B are among the top 50 proteins in Table 1. Note that keratins may be originated from surrounding tissue or environment. N: number of identified proteins. The proteins are listed in the order of reliability evaluated by the “score” and the “%Cov”. Score: A value indicating the reliability of the identification calculated from the accuracy of the amino acid sequence of the fragments, the intensity of peptide signals, coverage of the whole protein sequence, and number of fragments detected. % Cov: The ratio of amino acid number identified from the peptides to the whole sequence of the protein. Peptides (95%): The number of peptide fragments reliably identified for the protein