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Sequence alignment of prepro-orexin AAs in eight species, using the Clustal method (MEGALIGN, DNASTAR software). One insertion in zebra fish and two insertions in chicken are boxed. Genbank accession numbers were as follows, NP_001515.1 (human), NP_001181361.1 (monkey), NP_999321.1 (pig), NP_001159992.1 (bovine), NP_034540.1 (mouse), NP_037311.1 (rat), NP_001070860.1 (zebra fish), and NP_989516.1 (chicken).
