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Mean particle size of the lipid vesicles at pH 7.4 (A). Mean particle size (B) and surface potential (C) of the lipids 16a and 19a at different pH solutions. All measurements were performed at room temperature. Temperature-dependent steady-state fluorescence anisotropy measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) under the vesicle environment for 16a and 19a lipids within the range of 10–80 °C (D). The inset shows the Tm values of the lipids.
