Figure 6.

siRNA-mediated luciferase silencing using nanoparticles obtained via a templated or direct complexation method. The results are expressed as the percentage of luciferase expression relative to the average RLUs of the negative control (i.e., cells pre-transfected with the pGL3 plasmid DNA, without anti-Luc siRNA treatment) (n = 3). Statistical analysis (T-test, two-tailed) showed no significant differences between preparative methods in RAW macrophages, but a somehow better silencing efficiency of direct complexation (and of high-M_W chitosan) in HCT-116.