Fig. 4.

BDH2 reverses the epithelial–mesenchymal transition (EMT) in NPC cells. a Microarray assay: heatmap showing expression of 21 genes involved in EMT in BDH2-overexpressing 5–8F cells as compared with pCMV6-Entry–5–8F cells. b Relative expression of CDH1, EPCAM, SPARC and VIM analysed by real-time RT-PCR in BDH2–5–8F and pCMV6-Entry–5–8F cells. c Western-blot analysis of protein expression of E-cadherin, β-catenin, vimentin, SPARC and EPCAM in cells. GAPDH was used as an endogenous control. Data are mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001