Fig. 4.

PKD1 modulates the expression of important genes and proteins involved in enhanced cancer cell metabolism. a, b Western blot showing the expression level of key proteins associated with glucose metabolism, Glut-1 and HIF-1α following overexpression of PKD1 in HPAF-II and BxPC3 cells and c, d changes in real-time expression of Glut-1 and HIF-1α by quantitative real-time PCR in HPAF-II and BxPC3 cells. e Immunoblotting depicting the expression of mTOR and p-mTOR-S2448 in pancreatic cancer cells following PKD1 overexpression and (f) bars representing relative quantification of p-mTOR band intensity. g i–ii Immunoblotting depicting the mTOR and p-mTOR-S2448 protein expression levels in Panc-1 cells upon silencing of PKD1 and bars representing the relative quantification of p-mTOR band intensity. h Silencing PKD1 in Panc-1 cells having high expression of PKD1. The lower panel represents the densitometry analysis of band intensity. P-values are denoted by *P < 0.05. The blots were re-probed multiple times with β-actin being used as a protein-loading control