Fig. 5.

Silencing ASCT2 enhances DCA-induced ROS overproduction. a SCC15 and FaDu cells infected with a lentiviral vector expressing ASCT2 shRNA or a control vector were treated with either DMSO or DCA (10 mM, 48 h); then, the cells were analysed with an ROS detection kit and observed by a fluorescence microscope. Representative FaDu cells are shown. b SCC15 and FaDu cells treated with or without 1 mM H₂O₂ for 12 h or 24 h were lysed for the detection of ASCT2 by western blotting. c SCC15 and FaDu cells treated with or without 0.1 mM or 1 mM H₂O₂ for 24 h were lysed for the detection of ASCT2 by western blotting