Figure 1.

PPARγ silencing. (A) Uninfected peritoneal macrophages were obtained and, alternatively, transfected with two different PPARγ-siRNA during 72 h. Transfected or non-transfected cells were treated with HP₂₄ (100 μM) since 30 min before infection. These cells were infected for 48 h with T. cruzi (parasite:cell ratio 5:1). (B) Peritoneal macrophages from T. cruzi-infected mice (9 dpi) were obtained. These cells were alternatively transfected with two different PPARγ-siRNA for 72 h. Transfected or non-transfected cells were treated with HP₂₄ (100 μM) for 48 h. PPARγ expression was analyzed by RT-qPCR (six mice/group). Expression of PPARγ was determined by Western blot, and protein levels were normalized against α-actin. For both models, RNA and proteins were isolated 48 h after HP₂₄ treatment. Results represent the mean ± SEM of three independent experiments ^*P < 0.05 vs. uninfected cells, ^#P < 0.05 vs. T. cruzi-infected cells.