Figure 4.
PPARγ is required for the HP24 effects on PI3K/AKT/mTOR signaling pathway. Peritoneal macrophages were obtained and transfected with PPARγ-siRNA during 72 h. Transfected or non-transfected cells were treated with HP24 (100 μM) since 30 min before infection. Then, these cells were infected with T. cruzi for 30 min (parasite:cell ratio 5:1). Western blot analyses were carried out in cytosolic extracts, and p-AKT (Ser 473)/total AKT and p-p70 (Ser 411)/total p70 expression was analyzed. Results represent the mean ± SEM of three independent experiments. *P < 0.05 vs. uninfected cells, #P < 0.05 vs. T. cruzi-infected cells. ΔP < 0.05 vs. T. cruzi-infected and HP24-treated cells.