Figure 6.

HP₂₄ inhibits H₂O₂ production through PPARγ in T. cruzi-infected macrophages. Peritoneal macrophages were obtained and transfected with PPARγ-siRNA for 72 h. Transfected or non-transfected cells were treated with HP₂₄ (100 μM) since 30 min before infection. Then, these cells were infected with T. cruzi for 24 h (parasite:cell ratio 5:1). Sample histogram shows H₂O₂ generation measured by flow cytometry using 2′,7′dichlorofluorescein-diacetate (DCFDA). Ten thousand events were acquired for each group. Data are presented as the mean MFI ± SEM of three independent experiments. ^*P < 0.05 vs. uninfected cells, ^#P < 0.05 vs. T. cruzi-infected cells. ΔP < 0.05 vs. T. cruzi-infected HP₂₄-treated cells.