Figure 8.
HP24 inhibits the NF-κB pathway by PPARγ-dependent mechanisms. Peritoneal macrophages were obtained and transfected with PPARγ-siRNA for 72 h. Transfected or non-transfected cells were treated with HP24 (100 μM) since 30 min before infection. Then, these cells were infected for 30 min with T. cruzi (parasite:cell ratio 5:1). p65 expression was detected by confocal microscopy with a rabbit polyclonal anti-p65 antibody and a secondary goat anti-rabbit Alexa 647-labeled antibody. Cell nuclei were stained with DAPI. Representative microphotographs are shown. MFI represents nuclear p65. Scale bar: 5 μm. Results are expressed as the mean MFI of three independent experiments. *P < 0.05 vs. uninfected cells, #P < 0.05 vs. T. cruzi-infected cells. ΔP < 0.05 vs. T. cruzi-infected HP24-treated cells.