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. 2020 Jan 9;10:2963. doi: 10.3389/fimmu.2019.02963

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Copyright © 2020 Kiripolsky, Romano, Kasperek, Yu and Kramer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR expression is increased in splenic B cells from NOD.B10 mice in a Myd88-dependent manner and differs from that seen in salivary B cells. Spleens were isolated from female BL/10, BL/10^Myd88−/−, NOD.B10, and NOD.B10^Myd88−/− mice (n = 4 each) and SMG tissue was isolated from NOD.B10 females (n = 4 each). Flow cytometry was performed to assess expression of (A) TLR1, (B) TLR2, (C) TLR6, and (D) TLR4 on B220+ B cells. Significance was determined using a one-way ANOVA with the Tukey's multiple comparisons tests. Mean and SEM are shown (^*p ≤ 0.05, ^**p ≤ 0.01, ^****p ≤ 0.0001).