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. 2020 Jan 9;10:1663. doi: 10.3389/fpls.2019.01663

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Copyright © 2020 Zeng, Wen, Zhao, Wang and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/Cas9-induced gs3 mutants and the expression level of cell cycle-related genes. (A) Schematic representation of the GS3 genomic region. The two target sites of GS3 and PCR primers were marked in first and fifth exons. (B) The grain phenotype of gs3–9, gs3–21 and WT, and statistics for the average grain length. Bar = 1 cm. (C) The expression level of marker gene OsH1 and OsCYCT1 in WT, gs3–9 and gs3–21. Data are means ± SD from three biological replicates. Student’s t test, **P < 0.001, *0.001 < P < 0.05.