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The editing efficiency of CRISPR/Cas9 system in rice.
| Target Site | No. of Independent Plants | No. of plants with mutation | Mutation rate (%) | ||
|---|---|---|---|---|---|
| OsPIN5b-Site1 | 38 | 20 | 53 | ||
| OsPIN5b –Site2 | 38 | 16 | 42 | ||
| GS3-Site1 | 38 | 25 | 66 | ||
| GS3-Site2 | 38 | 24 | 63 | ||
| OsMYB30-Site 1 | 38 | 24 | 63 | ||
| OsMYB30-Site 2 | 38 | 22 | 58 | ||
| Target gene | No. of independent plants | No. of plants with mutation | Mutation rate (%) | ||
| OsPIN5b | 24 | 38 | 63 | ||
| GS3 | 25 | 38 | 66 | ||
| OsMYB30 | 26 | 38 | 68 | ||
| OsPIN5b/GS3/OsMYB30 | 8 | 38 | 21 | ||
