Table 2.
The putative off-target event in the ospin5b gs3 osmyb30 triple mutant lines.
| Target Site | Putative off-target site | The sequence of putative off-target site | No. of plants | No. of plants with mutations | Mutation rate (%) |
|---|---|---|---|---|---|
| OsPIN5b-Site1 | Chr3:9566074–9566052 | CCTCGAGCTCTGCAAGGCTTTGG | 8 | 0 | 0 |
| Chr1:19390027–19390049 | TTTCGAGCTGCGCAAGGCGCGGG | 8 | 0 | 0 | |
| OsPIN5b-Site2 | Chr7: 8747238–8747260 | CTCGTGTGCGCCAACGTTCTCGG | 8 | 0 | 0 |
| Chr3:24251589–24251567 | AACGTGCTCGTCAGCGTCCTCGG | 8 | 0 | 0 | |
| GS3-Site 1 | Chr8: 2495427–2495449 | GACGCGCTCCACCGCGCGCTCGG | 8 | 0 | 0 |
| Chr7:14816697–14816719 | AACTCGCTCAACCGCGAGAGGGG | 8 | 0 | 0 | |
| GS3-Site 2 | Chr9: 1832101–1832123 | TCGCGCCACCCCACACGAGGTGG | 8 | 0 | 0 |
| Chr7: 27472889–27472911 | TAACCCCGCACCGCATGAGGCGG | 8 | 0 | 0 | |
| OsMYB30-Site 1 | Chr3:13108929–13108951 | GTGGCGGAGAGCGACTGCACGGG | 8 | 6 | 75 |
| Chr5: 1475321–1475343 | CTGCGCGAGAGCGACTGCGCCGG | 8 | 0 | 0 | |
| OsMYB30-Site 2 | Chr4: 24538627–24538649 | TGACAAATGCTGCATGCCATGGG | 8 | 0 | 0 |
| Chr2:28909447–28909469 | CGTCAAATTCTGCATGACAGTGG | 8 | 0 | 0 |
The green font represented the PAM motif (NGG); the red font represented the mismatch bases.