FIG 4.

Representative dispersion responses of biofilms by P. aeruginosa PAO1, lapG::IS, cdrA::IS, and cdrB::IS strains. Biofilms were grown for 5 days in 5-fold-diluted VBMM in tube reactors. The absorbance of biofilm tube reactor effluents after the induction of dispersion is shown. (A) Absorbance of biofilm effluents after the induction of dispersion by glutamate. (B) Absorbance of biofilm effluents after the induction of dispersion by nitric oxide (NO). Sodium nitroprusside served as a source of nitric oxide. Dispersion assays were performed at least three times, with each biological replicate consisting of four technical replicates, but only representative dispersion profiles are shown.