FIG 6.

Lack of CdrA enhances dispersion upon induction of pslG gene expression. (A) Representative confocal images of 5-day-old biofilms by PAO1 and cdrA::IS strains harboring the empty vector pMJT-1 or expressing pelA or pslG. Images were acquired prior to and after treatment with arabinose to induce pelA or pslG gene expression. Scale bars, 100 μm. (B) Fold change in the biofilm biomass after the induction of dispersion by the addition of arabinose. Biofilm biomass prior to and after arabinose addition was quantitated by using COMSTAT. Experiments were performed in triplicate, with each biological replicate consisting of four technical replicates. **, significantly different (P < 0.05) relative to biofilms by PAO1 tested under identical conditions. ns, not significant. (C) Representative confocal images of 5-day-old biofilms by endA::IS and cdrA::IS strains harboring the empty vector pJN105 or expressing endA. Images were acquired prior to and after treatment with arabinose to induce endA gene expression. Scale bars, 100 μm. (D) Fold change in the biofilm biomass after the induction of dispersion by the addition of arabinose. The biofilm biomass prior to and after arabinose addition was quantitated using COMSTAT. Experiments were performed in triplicate, with each biological replicate consisting of four technical replicates. ns, not significant.