TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant genotype or descriptiona	Source or reference
Strains
Escherichia coli DH5α	F– ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+) phoA supE44 thi-1 gyrA96 relA1 tonA	Life Technologies
P. aeruginosa
PAO1	Wild-type strain	B. H. Holloway
PAO1::PBAD-pel	Chromosomal replacement of native promoter with araC-PBAD promoter, arabinose-inducible expression of pel genes	66
PAO1::PBAD-psl	Chromosomal replacement of native promoter with araC-PBAD promoter, arabinose-inducible expression of psl genes	67
endA::IS	PAO1 PA2749::ISlacZ Tetr	68
cdrA::IS	PAO1 PA4625::ISlacZ Tetr	68
cdrB::IS	PAO1 PA4624::ISphoA Tetr	68
lapG::IS	PAO1 PA1434::ISlacZ Tetr	68
Plasmids
pJN105	Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD Gmr	69
pJN-endA-V5	Arabinose-inducible expression of C-terminal V5/6×His-tagged endA (PA2749) cloned in pJN105, Gmr	32
pJN-PA2133	Arabinose-inducible expression of PA2133 cloned in pJN105, Gmr	56
pMJT-1	Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD Gmr	70
pMJT-pslG-V5	C-terminal V5-tagged PA3910 cloned into pMJT-1 XbaI and SacI; araC-PBAD Carbr	This study
pMJT-pelA-V5	C-terminal V5-tagged PA3909 cloned into pMJT-1 XbaI and SacI; araC-PBAD Carbr	This study

^aGm^r, gentamicin resistance; Tet^r, tetracycline resistance; Carb^r, carbenicillin resistance.