TABLE 2.
Primers used in this study
| Purpose and oligonucleotide | Sequence (5′–3′)a |
|---|---|
| Transposon insertional inactivation check | |
| lapG_FOR | TGGGACCTGGAGTCGATACT |
| lapG_REV | GCATCTTGGTCAGCAGGTC |
| cdrA_FOR | TCAACTGGAAGGGCTTCGAC |
| cdrA_REV | TCGTTGGTAGGGAAACTGGC |
| cdrB_FOR | CTGTACAGCCATCCCAGCTC |
| cdrB_REV | GAGGTTGTAGGTCTTGCCCC |
| Cloning | |
| pMJT1 MCS _f | GACCGCGAATGGTGAG |
| pMJT1 MCS _r | GAGCTGATACCGCTCG |
| Cloning into pMJT-1 for C-terminal V5-tagging | |
| pslG_For_NheI | gctagcATGGCACGTAAGGGACTCTAT |
| pslG_REV_SacI V5 | GCGCGCGCgagctcTCAcgtagaatcgagaccgaggagagggttagggataggcttaccCTCCCAGACCAGCATCTG |
| pelA_For_NheI | gctagcATGCGGTTCAGCAAGAAAGGA |
| pelA_REV_SacI _V5 | GCGCGCGCgagctcTCAcgtagaatcgagaccgaggagagggttagggataggcttaccGCAGACGAGTTGGCC |
a
Restriction sites are indicated in lowercase letters; the sequence of the V5 tag is underlined.