TABLE 2.

List of oligonucleotide primers used in this study

Name	Sequence (5′–3′)
Oligonucleotides for gene knockouts
Lon_KO_pKD4_fwd	GTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGTGTGTAGGCTGGAGCTGCTTC
Lon_KO_pKD4_rev	GATCGGCAATTACGTTGTCAGGAATCTCTTCCAGATCGCGTTTATTTTCGCATATGAATATCCTCCTTA
SulA_up_pKD3_fwd	AAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGTGTGTAGGCTGGAGCTGCTTC
SulA_down_pKD3_rev	GGATGTACTGTACATCCATACAGTAACTCACAGGGGCTGGATTGATTATGCATATGAATATCCTCCTTA
AcrB_up_pKD3_fwd	TTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGCATATGAATATCCTCCTTA
AcrB_down_pKD3_rev	TCAGCCTGAACAGTCCAAGTCTTAACTTAAACAGGAGCCGTTAAGACATGTGTGTAGGCTGGAGCTGCTTC
Oligonucleotides for site-directed mutagenesis
EcfolA_P21T_BglII_f	CGCCATGACGTGGAACCTGCCTGCAGATCTCGCCTGGTTTAAACGC
EcfolA_P21A_NaeI_f	CGCCATGGCGTGGAACCTGCCGGCCGATCTCGCCTGGTTTAAACGC
EcfolA_P21S_PvuII_f	CGCCATGTCGTGGAACCTGCCAGCTGATCTCGCCTGGTTTAAACGC
EcfolA_P21R_BglII_f	CGCCATGCGGTGGAACCTGCCTGCAGATCTCGCCTGGTTTAAACGC
folA_W30S_NheI_Rev	GGTGTTGCGTTTAAAGCTAGCGAGATCGGCAGGCAGG
folA_W30S_NheI_fwd	CCTGCCTGCCGATCTCGCTAGCTTTAAACGCAACACC
folA I94V AvaI F	GTGACGTACCCGAGATCATGGTGGTTGGCGGCGGTCGCG
folA I94F AvaI F	GTGACGTACCCGAGATCATGGTGTTTGGCGGCGGTCGCG
folA I94S AvaI F	GTGACGTACCCGAGATCATGGTGAGTGGCGGCGGTCGCG
folA I94N HpaI F	CCAGAAATCATGGTTAACGGCGGCGGTCGCGTTTATG
folA I94T AgeI F	CCAGAAATCATGGTGACCGGTGGCGGTCGCGTTTATG
folA I94M AvaI F	GTGACGTACCCGAGATCATGGTGATGGGCGGCGGTCGCG