TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| BL21(DE3) | F− hsdS gal λDE3 | Laboratory stock |
| MG1655 | E. coli K-12 λ− F− rph-1 | Laboratory stock |
| PK10882 | zdh-3632::cat, −26ATA−24 bp relative to sufA TSS changed to −26TAT−24 | 24 |
| PK11466 [BL21(DE3)Suf++] | BL21(DE3) but zdh-3632::cat, −26ATA−24 bp relative to sufA TSS changed to −26TAT−24 | This study |
| PK10028 | zdh-3632::cat | 24 |
| PK11465 [BL21(DE3)Suf+] | BL21(DE3) but zdh-3632::cat | This study |
| PK13230 | MG1655 but with sufB-3×FLAG-TAA-FRT-cat-FRT | This study |
| PK13231 | MG1655 but with sufS-3×FLAG-TAA-FRT-cat-FRT | This study |
| PK13232 | BL21(DE3) but with sufA/B-3×FLAG-TAA-FRT-cat-FRT | This study |
| PK13233 | BL21(DE3) but with sufS-3×FLAG-TAA-FRT-cat-FRT | This study |
| PK13235 | BL21(DE3) but with sufB-3×FLAG-TAA-FRT-cat-FRT derived from PK13230 | This study |
| PK13237 | BL21(DE3) but with sufS-3×FLAG-TAA-FRT-cat-FRT derived from PK13231 | This study |
| ZY-5 | Rhodobacter sphaeroides strain with deleted bchL used for Pchlide preparation | 33 |
| BW25993 | lacIq hsdR514 ΔaraBADAH33 ΔrhaBADL478 | 38 |
| PK10046 | BW25993 ΔiscSUA-hscBA-fdx::kan | This study |
| CAG18469 | MG1655 gua-26::Tn10(Tetr) | 41 |
| PK13354 | CAG18469 ΔiscSUA-hscBA-fdx::kan | This study |
| PK13343 | PK11466 ΔiscSUA-hscBA-fdx | This study |
| PK8202 | MG1655 kan-PydfZ-lacZ | 25 |
| PK12368 | BL21(DE3) kan-PydfZ-lacZ pRZ7404 | This study |
| PK12372 | PK11466 kan-PydfZ-lacZ pRZ7404 | This study |
| PK13351 | PK13343 kan-PydfZ-lacZ pRZ7404 | This study |
| NiCo21(DE3) | Commercial strain | New England BioLabs |
| Lemo21(DE3) | Commercial strain | New England BioLabs |
| C41(DE3) | Commercial strain | Lucigen |
| Rosetta2(DE3)pLysS | Commercial strain | EMD-Millipore-Novagen |
| BLR(DE3)pLysS | Commercial strain | EMD-Millipore-Novagen |
| BL21(DE3)Ai | Commercial strain | Thermo Fisher |
| BL21(DE3)codon plus | Commercial strain | Agilent Technologies |
| Plasmids | ||
| pIND4-3×FLAG(n) | kan, 3×FLAG | T. Donohue |
| pPK8629 | pIND4-3×FLAG(n) but with TAA inserted after 3×FLAG | This study |
| pPK8630 | pPK8629 but with FRT-cat-FRT cloned into BamHI and NdeI restriction sites | This study |
| pKD32 | FRT-cat-FRT | B. Wanner |
| pKD13 | FRT-kan-FRT | B. Wanner |
| pKD46 | Red recombinase expression plasmid | B. Wanner |
| pRZ7404 | Apr; HindIII-BamHI of fnr (−521 to +1115 of fnr) cloned into same sites of pBR322 | This study |
| BchL RSF-Duet1 | Plasmid used to overproduce BchL protein with an N-terminal 6×histidine tag and a 3C protease cleavage site | This study |
| BchB RSF-Duet1 | Plasmid used to overproduce BchB protein with an N-terminal 6×histidine tag and a 3C protease cleavage site | This study |
| BchN pET-Duet1 | Plasmid used to overproduce BchN protein | This study |