Table 1. Anti-DENV scFv effect on DENV infection, dissemination, and transmission rates.
DENV titers in WT, heterozygous and homozygous TADV-A (TADV-AHt and TADV-AHm, respectively), and wMel mosquitoes following a blood meal infected with one of four DENV serotypes are shown. DENV GE from mosquito midguts (at 4 or 14 dpi) and carcasses (14 dpi) of WT, TADV-A, and wMel (for DENV-2 only) mosquitoes were determined using RT-qPCR and calculated using previously published methods. Viral titers in saliva collected from WT, TADV-A, and wMel mosquitoes at 14 dpi were determined using TCID50 on Vero cells.
| DENV GE or viral titer on indicated dpi from specified tissue (proportion of mosquitoes with detected virus; percentage of mosquitoes with detected virus) | |||||
|---|---|---|---|---|---|
| DENV Serotype | Strain | 4 dpi | 14 dpi | ||
| Midgut– GE |
Midgut– GE |
Carcass– GE |
Saliva– viral titer |
||
| DENV-1 | WT | 5.70 x 103 (29/35; 83%) |
3.80 x 105 (35/40; 88%) |
4.80 x 105 (35/40; 88%) |
3.04 x 105 (35/40; 88%) |
| TADV-AHt | 5.00 x 101 (20/28; 71%) |
3.70 x 102 (25/30; 83%) |
2.40 x 102 (25/30; 83%) |
2.50 x 102 (25/30; 83%) |
|
| TADV-AHm | 0.00 x 101 (0/28; 0%) |
0.00 x 101 (0/28; 0%) |
0.00 x 101 (0/28; 0%) |
0.00 x 101 (0/28; 0%) |
|
|
DENV-2 |
WT | 4.25 x 104 (34/40; 85%) | 4.40 x 105 (40/46; 87%) |
5.35 x 105 (40/46; 87%) |
2.70 x 105 (38/46; 83%) |
| TADV-AHt | 8.20 x 101 (35/41; 85.4%) |
3.90 x 102 (26/30; 86.6%) |
6.70 x 102 (26/30; 87%) |
3.56 x 102 (25/30; 83.3%) |
|
| wMel | 4.75 x 101 (43/48; 90%) | 5.10 x 101 (38/48; 79%) |
6.45 x 101 (38/48; 79%) | 4.90 x 101 (35/48; 73%) |
|
| TADV-AHm | 0.00 x 101 (0/35; 0%) |
0.00 x 101 (0/30; 0%) |
0.00 x 101 (0/30; 0%) |
0.00 x 101 (0/30; 0%) |
|
|
DENV-3 |
WT | 1.80 x 104 (23/30; 77%) |
2.90 x 105 (29/35; 83%) |
3.50 x 105 (29/35; 83%) |
2.90 x 105 (29/35; 83%) |
| TADV-AHt | 3.60 x 101 (22/30; 73%) |
1.58 x 102 (20/30; 66%) |
1.60 x 102 (20/30; 67%) |
1.33 x 102 (20/30; 67%) |
|
| TADV-AHm | 0.00 x 101 (0/30; 0%) |
0.00 x 101 (0/30; 0%) |
0.00 x 101 (0/30; 0%) |
0.00 x 101 (0/30; 0%) |
|
|
DENV-4 |
WT | 3.25 x 103 (25/30; 83%) | 3.80 x 104 (25/32; 78%) |
1.50 x 105 (25/32; 78%) |
1.60 x 105 (25/32; 78%) |
| TADV-AHt | 3.40 x 101 (22/30; 73%) |
2.38 x 102 (19/28; 68%) |
2.95 x 102 (19/28; 68%) |
1.08 x 102 (19/28; 68%) |
|
| TADV-AHm | 0.00 x 101 (0/27; 0%) |
0.00 x 101 (0/28; 0%) |
0.00 x 101 (0/28; 0%) |
0.00 x 101 (0/28; 0%) |
|