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. 2020 Jan 16;15(1):e0227780. doi: 10.1371/journal.pone.0227780

Fig 4. Histological measurements of graft and scar size show no significant change with Shh treatment.

Fig 4

(A) Graph showing the total number of cells that makeup the graft for each heart. Black line is mean, error bars are SEM. (B,C) Representative images of hSC-CM grafts within the infarct scar of GFP-treated (B) or Shh-treated (C) hearts, visualized immunohistochemically via an antibody against β-myosin heavy chain (brown). Scale bars = 0.2 mm. (D) Quantification of scar size for each heart. Black line is mean, error bars are SEM. (E,F) Representative images of typical infarct scars stained with picrosirius red in hearts from GFP-treated (E) or Shh-treated (F) rats. Scale bars = 1 mm.