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. 2020 Jan 16;15(1):e0228055. doi: 10.1371/journal.pone.0228055

Fig 4. FHA44:PspA4Pro induces anti-PspA4Pro IgG but no protection against pneumococcal infection.

Fig 4

(A and B) BALB/c mice were immunized with one (A) or two (B) doses of FHA44:PspA4. FHA or PspA4Pro were used as negative or positive controls, respectively. Anti-PspA4Pro IgG were measured by ELISA. Circles represent individual data and lines represent the means for each group. Pools of sera from each group of mice were evaluated for the capacity to bind (C) or to induce complement deposition (D) on the surface of S. pneumoniae ATCC6303 by flow cytometry. Numbers indicate the medians of fluorescence intensity for each curve. (E) Intranasal challenge with S. pneumoniae ATCC6303 was performed in immunized mice and survival was followed for 15 days. *P<0.05; ****P<0.0001, One-way ANOVA with Tukey post-test (A and B) or Log-Rank survival curve with Mantel-Cox test (E).