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. 2020 Jan 6;18(1):e3000596. doi: 10.1371/journal.pbio.3000596

Fig 2. Intrinsic excitability is increased in L7-SK2 mice.

Fig 2

(A) PF strength was comparable in L7-SK2 and control mice, as assessed recording PF-EPSCs with increasing stimulation intensities, showing no significant differences (upper panel: representative traces, lower panel: PF-EPSC averaged per group at different stimulation intensities). (B) PF-EPSC PPR was comparable in L7-SK2 and control mice. It was calculated at increasing interpulse intervals as the ratio of the second EPSC over the first one, as a proxy of synaptic release probability (upper panel: representative traces, rescaled for each individual EPSC1; lower panel: PF-EPSCs averaged per group at different stimulation intensities). (C) CF-EPSCs were comparable in L7-SK2 and control mice (upper panel: representative traces, Vm = 0 mV; lower panel: CF-EPSCs averaged per group). (D) The number of CF inputs was comparable in both groups with a virtual absence of multi-innervation (upper panel: representative traces, Vm = −20 mV; lower panel: distribution of the number of CF inputs). (E) Purkinje cell excitability was increased in L7-SK2 mice, as assessed by the number of spikes evoked by a somatic depolarizing current injection of increasing amplitude (left panel: representative traces at 200 and 700 pA, right panel: spike count averaged per group per current amplitude). (F) Spontaneous simple spike firing rates in zebrin-negative (Z−; lobule III) and zebrin-positive (Z+; lobule X) Purkinje cells. *p < 0.05; **p < 0.01; ***p < 0.001. CF, climbing fiber; EPSC, excitatory postsynaptic current; PF, parallel fiber; PPR, pair-pulse ratio.