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. 2020 Jan 16;9:e50960. doi: 10.7554/eLife.50960

Figure 2. Ribo-Seq measurements reveal the architecture of viral coding regions.

Figure 2.

Examples of expression profiles of viral genes that contain novel ORFs conserved in HHV-6A and HHV-6B. Ribo-seq reads are presented in red and RNA-seq reads are presented in green. Canonical annotated ORFs are labeled by black rectangles, novel ORFs initiating at an AUG codon are labeled in blue, and novel ORFs initiating at a near-cognate start codon are labeled in orange. ORF sizes are written in gray. (A) U30 translation initiates at an AUG downstream of the annotated start codon. (B) A 32 amino acid (aa) upstream overlapping ORF (uoORF) is coded by the U48 transcript, initiates upstream of the U48 canonical ORF and partially overlaps it. (C) U36 locus contains two uORFs, as well as an out-of-frame iORF. (D) U84 locus contains an in-frame iORF which is a truncated version of U84, and a novel out-of-frame iORF.