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. 2020 Jan 16;9:e50960. doi: 10.7554/eLife.50960

Figure 8. Numerous iORFs and uORFs are conserved between betaherpesviruses.

(A) Correlation between the number of iORFs and uORFs of canonical ORFs in HHV-6A and HHV-6B (55 shared canonical ORFs in total). Dot size indicates the number of canonical ORFs with the indicated number of iORFs or uORFs in the two viruses. (B–C) Selected examples of novel internal or upstream initiation events that are conserved between HHV-6A and HHV-6B. Shown in black rectangles are canonical ORFs, in blue are novel ORFs initiating at an AUG codon, and in orange are novel ORFs initiating at a near-cognate start codon. ORF sizes are written in gray. The ribosome occupancy profiles are shown in red and the mRNA profile is shown in green (B) at U10 locus for both HHV-6A and HHV-6B and (C) at the U51 locus in HHV-6A and HHV-6B and its HCMV homolog U78. The gap in RNA reads in HHV-6B U51 is due to a base insertion relative to the reference, preventing read alignment to the region.

Figure 8.

Figure 8—figure supplement 1. Viral loci with conserved presence of multiple uORFs and iORFs.

Figure 8—figure supplement 1.

Ribo-seq reads (red) and RNA-seq (green) of several virus loci. Black rectangles represent canonical annotations, blue rectangles represent novel ORF initiating at an AUG codon and in orange rectangles represent ORFs initiating at a near-cognate start codon. ORF sizes are written in gray. (A) Multiple in-frame iORFs within U70 in HHV-6A and HHV-6B. (B) Multiple uORFs upstream of U32 ORF in HHV-6A and HHV-6B. LTM ribosome density profiles resemble the Harr ribosome density profiles and are not presented.
Figure 8—figure supplement 2. Synteny conservation of uORFs and iORFs between HHV-6 and HCMV.

Figure 8—figure supplement 2.

Correlation between the number of iORFs and uORFs of canonical HCMV and HHV-6 ORFs (26 canonical main ORFs in total). Dot size indicates the number of canonical ORFs with the indicated number of iORFs or uORFs in the two viruses. (A) HHV-6A and HCMV uORFs, (B) HHV-6B and HCMV uORFs, (C) HHV-6A and HCMV iORFs, and (D) HHV-6B and HCMV iORFs.