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. 2020 Jan 16;15(1):e0227624. doi: 10.1371/journal.pone.0227624

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© 2020 Chen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) The infarct area was observed by TTC staining of brain slices from each cohort. The viable tissue is stained red by TTC staining, but the infarcted area remains unstained, exhibiting pale yellow/white color. (B) Quantitative evaluation of percent infarction volume in different cohorts of mice was observed at 76 h after MCAO. The LRIP treatment group had significantly lower infarction volume compared with the sham group. The infarction volume was similar in cohort 2 and cohort 4, suggesting that the reduction in infarction volume was independent of the anesthetic used. Data are presented as mean ± SD. n = 6–11, *p < 0.05.