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. 2020 Jan 10;7:442. doi: 10.3389/fbioe.2019.00442

Figure 3.

Figure 3

IB formation upon overexpression of chaperones. (A) IB sedimentation assay on cells co-transformed with pIBA-ssTorA/TrxA and pLysTac-TorD as described in the legend to Figure 1. Thirty minutes prior to induction of expression of ssTorA/TrxA IPTG was added to half of the culture to induce overexpression of TorD (lanes 4–6). The other half of the culture was left without IPTG (lanes 1–3). (B) IB sedimentation assay on cells co-transformed with pIBA-ssTorA/TrxA and either pOFX-tac1-DnaK/DnaJ (lanes 4–6) or pOFX-tac-MBP (lanes 7–9). Thirty minutes prior to induction of expression of ssTorA/TrxA IPTG was added to the respective cultures to induce overexpression of DnaK/DnaJ (lanes 4–6) or MBP (lanes 7–9). Cells co-transformed with pIBA-ssTorA/TrxA and the empty vector pOFX-tac1 served as a negative control for co-overexpression (lanes 1–3). Bands representing ssTorA/TrxA fusions of interest are indicated (♦). Molecular mass (kDa) markers are indicated at the left side of the panels.