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. 2020 Jan 10;7:442. doi: 10.3389/fbioe.2019.00442

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Copyright © 2020 Jong, ten Hagen-Jongman, Vikström, Dontje, Abdallah, de Gier, Bitter and Luirink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IB formation upon incremental deletion from the N-terminus of ssTorA. (A) SDS-PAGE/Coomassie staining analysis on cells overexpressing TrxA fused to wild-type ssTorA or an ssTorA variant carrying any of the indicated amino acid deletions. Expression of TrxA not fused to any ssTorA moiety is labeled Δ1–39. (B) IB-sedimentation assay as described in the legend to Figure 1 on cells expressing a selection of the ssTorA/TrxA fusions from A. Bands representing signal sequence/TrxA fusions of interest are indicated (♦). Molecular mass (kDa) markers are indicated at the left side of the panels.