FIG 7.

Effect of phosphorylation on the subcellular localization of sortilin. Subcellular fractionation of HEK cells transfected with either wt sortilin or S15D mutated sortilin (HEK/sortilin and HEK/sortilin S15D cells, respectively) was performed by centrifugation in a discontinuous iodixanol density gradient. Twenty-four fractions were collected by ultracentrifugation and subsequently analyzed by Western blotting using mouse antisortilin (catalog number 612100; BD Biosciences). Bars indicate the distribution of fractions with the largest amount of sortilin.