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. 2020 Jan 16;40(3):e00357-19. doi: 10.1128/MCB.00357-19

FIG 3.

FIG 3

LINC01094 absorbed miR-224-5p. (A) The levels of different miRNAs in sh-NC or sh-LINC01094#2-transfected cells were certified by RT-qPCR (**, P < 0.01 [Student t test]). (B) The levels of miR-224-5p in normal cells and ccRCC cells were tested by using RT-qPCR (*, P < 0.05 [one-way ANOVA]). (C) After transfection by miR-224-5p mimic or NC mimic in ACHN and 786-P cells, RT-qPCR analysis detected miR-224-5p expression (**, P < 0.01 [Student t test]). (D and E) A luciferase reporter assay (with a schematic) and an RIP assay confirmed the interplay of LINC01094 with miR-224-5p (*, P < 0.05; ***, P < 0.001 [Student t test]). (F) AN RNA pulldown assay assessed the enrichment of LINC01094 in the bio-NC, bio-miR-224-5p-WT, and bio-miR-224-5p-MUT groups (**, P < 0.01 [one-way ANOVA]). (G) An RT-qPCR was used to determine the LINC01094 levels in transfected ACHN and 786-P cells (*, P < 0.05 [Student t test]).